英语翻译，请高手帮忙修改（中译英是否正确）
基于立场的情感是后天获得的,引用现有的理论观点和实验结果,本文检视与分析的组成部分的情感的体验和感官感觉(例如疼痛、饥饿、味觉、嗅觉等)的内容,并相信他们是所有的化合物的感情由认知表现和影响表示。并提出的假设,即“情感体验的核心部件来源于条件反射的感官感觉”影响的组成部分,根据分离性的认知表现和影响表现在在情感的体验和感官的感觉,和相同的神经基础的影响表现在在之间情感的体验和感官的感觉。它也讨论了具体表现的认知表现和影响表现在情感的体验和相关的心理现象,并认为客观化的影响是一个重要的心理机制在情感体验。
由于SMART技术是利用逆转录酶内源的末端转移酶活性，只要单管，一步即可完成，不需要额外的cDNA抽提纯化或者沉淀，或者额外的酶反应，只需要少至25ng的mRNA或者50ng的Total RNA就可以得到高质量、高产量的cDNA库，更重要的是得到的cDNA能够代表原有样品中的mRNA的丰度，因此在本实验中把它直接用于构建绒山羊（Capra hircus）毛囊兴盛期皮肤组织的cDNA质粒文库。Trizol Reagent（GIBCO/BRL）分离RNA后，需要提取mRNA，这需要用Oligotex（QIAGEN），提取步骤如下：以锚定引物反转录合成cDNA第一链作为模板链，以两个锚定引物，用长链PCR扩增全长cDNA双链。用SfiⅠ酶切去除小片段后的CHROMA SPIN-400，连接到SfiⅠ消化的pBluescriptⅡ SK（带有SfiⅠA和B两个位点）质粒载体中，转化E.coli 5a，库容量为1.8×105clones。通过对文库中克隆子的序列测定和生物信息学初步分析，并且用B lastX及B lastN软件进行同源对比，得到1200多条EST序列，本篇文章就从中选取20多条进行分析，辽宁新品系绒山羊cDNA文库以及EST文库的成功构建,为研究辽宁新品系绒山羊功能基因和蛋白质组学奠定了基础。
New Strain of Liaoning cashmere goat skin, hair follicles hugely popular for a cDNA library construction and EST sequence analysis
Abstract: The SMART technology is the use of reverse transcriptase endogenous terminal transferase activity, as long as the single-tube, one step can be completed, no additional purification or precipitation of the cDNA extraction, or an additional enzymatic reactions, just as little as 25ng of the Total RNA or 50ng of mRNA can get high-quality, high-yield cDNA library, more importantly, to be able to represent the original sample of cDNA of mRNA abundance, so in this experiment used to construct it directly to cashmere goat (Capra hircus) follicle hugely popular skin tissue cDNA plasmid library. Trizol Reagent (GIBCO / BRL) separation of RNA, you need to extract the mRNA, which requires using Oligotex (QIAGEN), extraction steps are as follows: the anchor primer first-strand cDNA synthesis of reverse transcription as a template chain, with two anchor primers, using PCR amplification of long-chain full-length double-stranded cDNA. Digested with Sfi Ⅰ after the removal of small fragments CHROMA SPIN-400, connect to the Sfi Ⅰ digested pBluescript Ⅱ SK (with Sfi Ⅰ A and B 2 sites) plasmid vector and transformed into E.coli 5a, storage capacity of 1.8 × 105clones . Library clones by sequence determination and bio-informatics preliminary analysis, and with the B lastX and B lastN software homologous comparison, received more than 1,200 of EST sequences, this article will select from more than 20 analysis, Liaoning New strain cashmere goats EST library cDNA library as well as the successful construction of new lines for the study of Liaoning cashmere goats functional genomics and proteomics basis.
Good afternoon.White Swan.May I help you?
B: 我叫大卫。霍金斯。我下星期二将前入广州参加广交会。不知可否在贵宾馆订间房？
This is David Hawkins.I'm going to GuangZhou to attened Canto Fair on next Tuesday.May I reservation in your hotel?（改为 May I book one room in your hotel?)
A：实在抱歉，我们所有的房间都订满了。
Sorry,the rooms are booked up.
B：没关系。再见。
It doesn't matter.Byebye.
C：你好！这里是广州国际大酒店。能为你效劳吗？
Hello,Guangzhou International Hotel.May I help you?
B：我叫大卫。霍金斯。我想在贵宾馆订间房，不知你们是否还有空房？
This is David Hawkins. I'd like to book a room in your hotel.Do you have any vacancies?
C：请稍等，我查一查。霍金斯先生，我们只剩一间单人房和一间双人房。
Just a second（wait one moment 更好 ).Let me check.Mr Hawkins,we only have one single room and one double room.
B：请问单人房多少钱一晚？
How much is a(the ) single room （each night）?
C：$200.
$200
B:房里有些什么设备？
What kinds of facilities at （in）the room?
C：房里有中央空调，电话，电视等。
There is（are） air conditioner,phone,TV set.
B：有电脑吗？
Is there a computer?
C：对不起，没有。但是你可以租。
It's not（Sorry， no have）,but you can rent.
B：好吧，我就订那间单人房。
Ok,I'd like to book the single room.
C：你什么时候需要房间，打算住多久？
When would you like to stay here?（When you will come and how long you will stay ?)
B：我下星期一下午到达广州，从星期一住到星期五，一共五个晚上。
I'll arrive on afternoon of next Monday and leave on Friday,five days in all.
C：好的，一间单人房，住五个晚上，从周一到周五。
Ok,a single room,five days in all,starting (Next) Monday to Friday.
B：谢谢。
Thanks.
C：不客气。
Not at all.
2.A：您好，客房服务，要帮忙吗？
Hello,Room Service,may I help you?
B：是的，我叫杰克。格林，住2245房。我有些衣服要洗。
This is Room 2245.I have some close(clothes) need to wash.
A：先生，请把您要洗的衣服放到塑料袋里，然后填份洗衣表格。有人会去取。
Sir,put your close(clothes) in the plastic(poly) bag,and fill out a profile(and fill the sheet).We'll collect.(we will go to fetch)
B：塑料袋在哪儿？
Where's plastic(poly) bag?
A：塑料袋和洗衣表格在您梳妆台右边的第二个抽屉里。
The (poly bag) and landry list are contained(去掉cantained） in your dresser right the second drawer.（in the second drawer on the right of your dressing table)
B：我的大衣要干洗，衬衫要水洗，西装要烫。这需要多长时间？
The overcoat is dry-cleaned,the skirt(shirt) is water washed,the suit is pressed(need iron).
A：我想半天就可以了。还有什么吗？
Half of the day(Half day ).Anything else?
B：请你明天早上6点钟用电话叫醒我，好吗？
I need a wake-up call tomorrow at 6:00.
A：好的。
OK.
B：能否给我送些苹果，香蕉和今天的（中国日报）？
Would you send me some apple,banana and today's China Daily?
A：可以。我们一刻钟后给你送去。
Sure,we'll bring it to you in 15 munites.
B：谢谢。
Thanks.
1. 第一段广交会写错了.应该是"Canton Fair".
2. "May I reservation in your hotel"错了,"reservation"是名词而非动词.可改为"make a reservation"
3. "There is air conditioner,phone..."应该是"There are"
4. "starting Monday to Friday"应该在"starting"后加个"from"
5. 第二段的"close"应该是"cloth"的误写.
6. "The overcoat is dry-cleaned,the skirt is water washed,the suit is pressed"中,"is"应该用"to be",衬衫应该是"shirt"而非"skirt".
您好，客房服务，要帮忙吗？
Hello,Room Service,may I help you?
B：是的，我叫杰克。格林，住2245房。我有些衣服要洗。
This is Room 2245.I have some close need to wash.
A：先生，请把您要洗的衣服放到塑料袋里，然后填份洗衣表格。有人会去取。
Sir,put your close in the plastic bag,and fill out a profile.We'll collect
B：塑料袋在哪儿？
Where's plastic bag?
A：塑料袋和洗衣表格在您梳妆台右边的第二个抽屉里。
The plastic and landry list are contained in your dresser right the second drawer.
B：我的大衣要干洗，衬衫要水洗，西装要烫。这需要多长时间？
The overcoat is dry-cleaned,the skirt is water washed,the suit is pressed.
A：我想半天就可以了。还有什么吗？
Half of the day.Anything else?
B：请你明天早上6点钟用电话叫醒我，好吗？
I need a wake-up call tomorrow at 6:00.
A：好的。
OK.
B：能否给我送些苹果，香蕉和今天的（中国日报）？
Would you send me some apple,banana and today's China Daily?
A：可以。我们一刻钟后给你送去。
Sure,we'll bring it to you in 15 munites.
B：谢谢。
Thanks.
C：房里有中央空调，电话，电视等。
There is air conditioner,phone,TV set.后面应该加上and soon
实际上一句汉语用英语可以译出很多种不必太按书上的去做
英语翻译，请高手帮忙修改（中译英是否正确）视频
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